 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Sonication-Assisted Agrobacterium-mediated Gene Transformation of Oil Palm Secondary Somatic Embryo) ผู้เขียน: Sureerat Yenchon, Sompong Te-chato สื่อสิ่งพิมพ์:pdf AbstractThe infl uence was investigated of the hygromycin concentration on the survival rate of oil palm secondary somatic embryos (SSEs) and sonication-assisted Agrobacterium-mediated transformation (SAAT). The results showed that 20 mg.L-1 hygromycin could completely inhibit growth of SSEs, suitable for selection of transformed SSEs. The transient ?-glucuronidase (gus) expression was affected by the different period of SAAT which was signifi cantly enhanced by increasing the period of SAAT. However, the longer exposure to SAAT caused a decrement in the frequency of plantlet regeneration. The highest transformation effi ciency was obtained when the SSEs were sonicated for 5 min. Polymerase chain reaction and Southern blot hybridization analysis confi rmed the presence of the reporter genes in the transformed plantlet genome. |